First, evaluate the part of the spectrum you’re measuring. Cuvette materials are chosen from these alternatives. This will save you money if you’re on a limited budget.
“VIS” refers to a person’s capacity to perceive light. This visible light spectrum can be comprehended by humans. NASA’s VIS cuvette passes 380 to 700 nm light. Any material cuvettes may be created cheaply such as UV-vis Cuvettes.
Actual Source NASA
Materials that can be used to make vis cuvettes?
This table lists visible cuvette materials and their transmission ranges.
|Name||Range of Use||Spectrum Range That Can Be Used|
|UV Quartz||UV-VIS||190 to 2,500 nm|
|IR Quartz||UV-VIS-IR||250 to 3,500 nm|
|Polystyrene||VIS||340 to 900 nm|
|UV Plastic||UV-VIS||220 to 900 nm|
|Acrylic||VIS||285 to 900 nm|
|Optical Glass||VIS||340 to 2,500 nm|
Can I do fluorescence with the vis cuvette?
Definitely! Using any vis cuvette with four sides, you can get a reading for fluorescence. When the two sides of a vis cuvette are cloudy, having two sides that are clear doesn’t help much.Vis measurements are done using disposable cuvettes since they’re cheap and simple to clean. The one hundred cuvettes that come in a package don’t need to be cleaned or washed after use because they are disposable. Fantastic!
Use your disposable cells as much as possible by following these simple steps:
- Before resetting the machine, be sure two or three cuvettes have identical transmissions. Plastic cuvettes made in different batches can hold different amounts of liquid.
- Look for scratches and other flaws on the optical surfaces of plastic cells.
- As a safety measure, cuvettes should always have their lids on when they are not being used.
Handle the pieces that are frosted, but try not to touch the clear ones.
- If you want to use the sample again, you can use a micropipette to get the droplets out of the corners of the cuvette. You’ll be able to use the sample again.
- Cuvettes made of plastic should only be used once before being thrown away.